Position title: Professor of Pharmaceutical Sciences and Chemistry
Madison, WI 53706
- Research Website
- Mecozzi Group
- B.S./M.S. (Laurea) 1988, First University of Rome “La Sapienza” (Italy)
- Ph.D. 1996, California Institute of Technology
- Postdoctoral Associate, Scripps Research Institute
Our research efforts focus on fundamental studies of non-covalent interactions and the application of the resulting findings to biochemical and biomedical problems. We use the predictive value of advanced computational techniques in conjunction with the power of chemical synthesis to generate molecules that are able to recognize each other through intermolecular forces. The study of these complexes allows us to explore a variety of different chemical and biological phenomena. These include the nature of specific intermolecular forces, the origin of the peculiar fluorous phase behavior in highly fluorinated materials, targeted drug delivery through semifluorinated functional materials, and the recognition properties of RNA.
The Fluorous Phase
The introduction of fluorine substituents into an organic molecule can radically change the physico-chemical properties of that molecule. High performance materials and polymers, vectors for drug delivery, anesthetics, fluorine-containing drugs, perfluorinated solvents for organic reactions are only a few examples of the practical uses of fluorinated molecules. Extensive perfluorination of organic molecules generates a new phase of liquid matter known as the fluorous phase. This phase does not mix with either polar or non-polar hydrogenated phases. The formation of a fluorous phase is at the basis of the unusual behavior of heavily fluorinated molecules and polymers. While the applications and the uses of fluorinated compounds are constantly increasing, the origin of their unusual properties is currently not completely understood. It is not known what exactly drives the formation of a fluorous phase.
We are currently investigating the nature of the fluorous phase by synthesizing and analyzing several self-assembling fluorinated amphiphilic molecules bearing water solubilizing groups and variously fluorinated functionalities.
We are also taking advantage of the large energetics associated with the formation of a fluorous phase to generate thermodynamically stable (micelles) and kinetically stable (nanoemulsions) nanoaggregates that can be used for the efficient and selective intravenous delivery of general anesthetics to the brain. To this purpose, we have designed and synthesized a number of novel, semifluorinated di- and triblock copolymers. The use of specific FDA-approved additives allows us to switch from thermodynamic to kinetically stable aggregates with the corresponding change in the delivery properties of the nanoparticles. We use a similar approach for the selective delivery of powerful anticancer therapeutics.
We are also performing molecular dynamics simulations on mixtures involving water, hydrocarbons, and a variety of fluorinated molecules. The purpose of these simulations is to establish first what kind of intermolecular forces can explain the formation of three phases and then identify the minimum number of fluorine atoms that are necessary for generating a fluorous phase under different conditions.
The Nature of Intermolecular Interactions
We focus our studies on understanding the basic principles behind weak, yet biologically and energetically significant non-covalent forces. Our most recent studies have focused on using the recognition events between enzymes and their inhibitors to study the energetics of specific intermolecular interactions. This approach can be fruitfully used to study a variety of different intermolecular interactions and, at the same time, to achieve a deeper understanding of the enzymes’ inner machinery. These experimental studies are complemented by high level ab initio calculations on simple complexes.
Short RNA-Small Molecules Complexes
Our research in this field focuses on finding the minimal RNA sequences able to bind small organic molecules with high selectivity and affinity. Our own approach starts from the analysis of the crystal structure of RNA aptamer-small molecule complexes. We then computationally model these complexes to study the effect of nucleotide deletion and spatial rearrangement on the overall energetics. This approach allows us to computationally design short RNAs that are able to specifically bind small molecules. Using this methodology, we have been able to identify the minimum sequence requirements for the binding of molecules like theophylline, flavin mononucleotide, and aminoglycosides. All computational results have been confirmed experimentally. The current emphasis in this project is on the study of ribonucleoprotein complexes. We are also pursuing a dynamic combinatorial approach to RNA recognition.