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Chemical Biology Seminar – Dr. Eric Schreiter, Howard Hughes Medical Institute, Janelia Research Campus
March 2 @ 11:00 am - 12:00 pm
Dr. Eric Schreiter
Howard Hughes Medical Institute, Janelia Research Campus
Chemical Biology, Seminar
CHEMIGENETIC INDICATORS OF NEURONAL ACTIVITY
Fluorescent indicators of cell physiology have become ubiquitous tools for biology, especially neuroscience, because they allow sensitive monitoring of signals with high spatiotemporal precision over large areas of tissue. Many of the most popular indicators, like the calcium indicator GCaMP, are engineered from fluorescent proteins like GFP. During the last ~3 years, we have worked to develop new scaffolds for fluorescent indicators that combine engineered protein sensor domains with small molecule fluorophore reporters that have better photophysical properties than fluorescent proteins, such as higher brightness, improved photostability, and red-shifted emission. We call these hybrid protein-small molecule sensors ‘chemigenetic’ indicators. I will present the development and application of several such chemigenetic indicators, highlighting their advantages over existing sensors.
FOR MORE INFORMATION, CONTACT: ALISA GRADNEY AT AGRADNEY@WISC.EDU