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McElvain Lecture: Analytical Chemistry Seminar – Prof. Julia Laskin, Purdue University

March 4, 2021 @ 12:05 pm - 1:00 pm

Event Presenter:

Prof. Julia Laskin

Institution:

Purdue University

Categories:

Analytical Chemistry, Seminar

AMBIENT IMAGING OF BIOLOGICAL SAMPLES USING NANOSPRAY DESORPTION ELECTROSPRAY IONIZATION (NANO-DESI) MASS SPECTROMETRY

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Mass spectrometry imaging (MSI) is a powerful technique for studying  the localization of lipids, metabolites, and proteins in biological samples. We have developed nanospray desorption electrospray ionization (nano-DESI), an ambient ionization technique that relies on localized liquid extraction of analyte molecules from the sample into a liquid bridge formed between two glass capillaries. The extracted analytes are transferred to a mass spectrometer inlet and ionized by electrospray ionization. Nano-DESI enables quantitative imaging of biomolecules in fully hydrated samples with minimal or no sample pre-treatment. Simultaneous imaging of sample topography and chemical composition has been achieved by coupling nano-DESI MSI with shear force microscopy. This multimodal imaging approach has enabled imaging of living microbial communities with a complex topography and high-spatial-resolution imaging of tissue sections providing an unprecedented depth of chemical information in each imaging experiment. Recent developments in the nano-DESI MSI instrumentation have enabled quantitative imaging of lipids and metabolites in tissues with high sensitivity and spatial resolution down to 8-10 microns using finely pulled capillaries. Furthermore, we have developed a microfluidic nano-DESI probe, which greatly simplifies the experimental setup and demonstrates similar performance to the capillary probe. In this talk, I will introduce the technique, highlight the most important developments of nano-DESI MSI instrumentation, and discuss several applications of nano-DESI MSI.

FOR MORE INFORMATION, CONTACT: ALISA GRADNEY AT AGRADNEY@WISC.EDU

Seminar Poster Laskin, Julia

Details

Date:
March 4, 2021
Time:
12:05 pm - 1:00 pm
Event Categories:
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